Classification of tyrosine kinases fromDictyostelium discoideumwith two distinct, complete or incomplete catalytic domains
نویسندگان
چکیده
منابع مشابه
Classification of tyrosine kinases from Dictyostelium discoideum with two distinct, complete or incomplete catalytic domains.
Two new kinases of Dictyostelium discoideum were identified by screening of a (lambda)gt11 expression library with a phosphotyrosine specific antibody. Amino-acid sequences derived from cDNA and genomic clones indicate that DPYK3 is a protein of 150 kDa and DPYK4, a protein of 75 kDa. The C-terminal fragments of each protein were produced in Escherichia coli and shown to be autocatalytically ph...
متن کاملTyrosine phosphorylation of protein kinase CK2 by Src-related tyrosine kinases correlates with increased catalytic activity.
Casein kinase-2 (CK2) is a pleiotropic and constitutively active serine/threonine protein kinase composed of two catalytic (alpha and/or alpha') and two regulatory beta-subunits, whose regulation is still not well understood. In the present study, we show that the catalytic subunits of human CK2, but not the regulatory beta-subunits, are readily phosphorylated by the Src family protein tyrosine...
متن کاملTwo Catalytic Domains Are Required for Protein
Received for publication, June 8, 2005, and in revised form, November 3, 2005 Published, JBC Papers in Press, November 6, 2005, DOI 10.1074/jbc.C500241200 Yu Zhang, Benoit Gilquin, Saadi Khochbin, and Patrick Matthias From the Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, P. O. Box 2543, Maulbeerstrasse 66, 4058 Basel, Switzerland and the INSERM U309, Insti...
متن کاملMechanisms of Activation of Receptor Tyrosine Kinases: Monomers or Dimers
Receptor tyrosine kinases (RTKs) play essential roles in cellular processes, including metabolism, cell-cycle control, survival, proliferation, motility and differentiation. RTKs are all synthesized as single-pass transmembrane proteins and bind polypeptide ligands, mainly growth factors. It has long been thought that all RTKs, except for the insulin receptor (IR) family, are activated by ligan...
متن کاملAnchor-based classification and type-C inhibitors for tyrosine kinases
Tyrosine kinases regulate various biological processes and are drug targets for cancers. At present, the design of selective and anti-resistant inhibitors of kinases is an emergent task. Here, we inferred specific site-moiety maps containing two specific anchors to uncover a new binding pocket in the C-terminal hinge region by docking 4,680 kinase inhibitors into 51 protein kinases, and this fi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1996
ISSN: 0014-5793
DOI: 10.1016/0014-5793(96)01053-8